Effects of Radix Achyrandii Polysaccharide on Cartilage Morphology of Knee Osteoarthritis in Obese Mice Induced by High-fat Diet

Authors

  • Jiaqi Zhang
  • Yaxuan Deng
  • Jiangyuan Chen
  • Ying Zhai
  • Fuyan Wang
  • Fahu Yuan

DOI:

https://doi.org/10.54097/64zd5m60

Keywords:

Radix achyrandii polysaccharide, High fat; Blood lipid; Osteoarthritis.

Abstract

Objective: To study the pathological changes of articular cartilage in the process of osteoarthritis induced by high-fat diet and the mechanism of Radix achyrandii polysaccharide on osteoarthritis cartilage, and to explore the protective effect of Achyrannosus achyrannosus polysaccharide on knee osteoarthritis cartilage, so as to provide effective experimental evidence for clinical treatment of knee osteoarthritis. Methods: OA model induced by anterior cruciate ligament transection was established in obese rats induced by high-fat diet. Thirty C57BL/6 J mice were randomly divided into normal diet group (CON), high fat diet group (OA) and high fat diet plus Radix achyrandii polysaccharide (RAP). The CON group was fed with a basal diet, and the OA and RAA groups were fed with a high-fat diet. After 8 weeks of feeding, the RAP group was treated with polysaccharide (200 mg/kg·d) by gavage once a day for 4 weeks, and the blank group and the model group were given the same volume of normal saline. At the end of the experiment, the serum levels of TG, TC, HDL-C and LDL-C were detected by biochemical methods. The activity of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were measured by ELISA. H&E staining was used to observe the pathological changes of knee cartilage. Results: Compared with the CON group, the OA group had significantly higher serum levels of TG, TC, LDL-C, MDA, TNF-α and IL-1β, and significantly lower serum HDL-C level and SOD activity. The results of histopathological examination showed that there were pathological changes of cartilage tissue damage in OA group. Compared with the model group, the related indicators of the RA group were improved to varying degrees. Conclusion: Radix achyrandii polysaccharide can protect articular cartilage by improving OA cartilage damage caused by anterior cruciate ligament transection in obese rats, regulating blood lipid related indicators, and inhibiting inflammatory response and oxidation reaction.

Downloads

Download data is not yet available.

References

[1] Arashi Shahid, Aricia Jieqi Thirumaran, Robin Christensen, Venkatesha Venkatesha, Marius Henriksen,Jocelyn L. Bowden, David J. Hunter.(2024).Expression of Concern; Comparison of weight loss interventions in overweight and obese adults with knee osteoarthritis: a systematic review and network meta-analysis of randomized trials.Osteoarthritis and Cartilage (11), 1513-1513.

[2] Shifan Zhao,Yijiong Tao,Linjie Huang,Hualei Xue,Rong Chen,Xiao Li.Mi Wang.(2024).Effects of polysaccharide from Pueraria lobata on osteoarthritis in rats.International Journal of Biological Macromolecules(P3),134901-134901.

[3] Yanzhi Liu,Rui Lin,Haiping Fang,Lixian Li,Min Zhang,Lujiao Lu.Liao Cui.(2024).Sargassum polysaccharide attenuates osteoarthritis in rats and is associated with the up-regulation of the ITGβ1-PI3K-AKT signaling pathway. Journal of Orthopaedic Translation176-190.

[4] Amany Abd Elaal Aiad, Sahar Mohamed El Haggar,Amal Mohamed El Barbary, Dalia Refat El Afify.(2024).Metformin as adjuvant therapy in obese knee osteoarthritis patients..Inflammopharmacology(4),2349-2359.

[5] Dolatkhah Neda, Toopchizadeh Vahideh, Barmaki Sahel, Salekzamani Yagoub, Najjari Afsaneh, Farshbaf Khalili Azizeh Dolati Sanam.(2022).The effect of an anti-inflammatory in comparison with a low caloric diet on physical and mental health in overweight and obese women with knee osteoarthritis: a randomized clinical trial..European journal of nutrition(2),659-672.

[6] Alanazi J,Unnisa A,Patel R D,Itumalla R,Alanazi M,Alharby T N, Mohamed O A.(2022).Prevalence of cardiovascular disease and osteoarthritis in obese population of Hail region, Saudi Arabia..European review for medical and pharmacological sciences(19),7161-7168.

[7] Oo Win Min,Mobasheri Ali , Hunter David J.(2022).A narrative review of anti-obesity medications for obese patients with osteoarthritis..Expert opinion on pharmacotherapy (12), 1381-1395.

[8] Obiegbu Obinna.(2022).Osteoarthritis of the knee joint in the obese patient: Do metabolic factors play a role?.Sahel Medical Journal(2),57-60.

[9] Neslihan DÜLGER, Mehmet AKMAN , Barış YILMAZ. (2022). The Effect of Weight Loss on Osteoarthritis Symptoms in Obese Patients with Osteoarthritis.Bezmialem Science (2), 206-211.

[10] Xu Chao,Ni Su,Zhuang Chao,Li Chenkai,Zhao Gongyin, Jiang Shijie, Wang Yuji.(2021).Polysaccharide from Angelica sinensis attenuates SNP-induced apoptosis in osteoarthritis chondrocytes by inducing autophagy via the ERK1/2 pathway.Arthritis Research & Therapy(1),47-47.

[11] Castro Rondinelle Ribeiro, Feitosa Judith Pessoa Andrade,da Cunha Pablyana Leila Rodrigues, da Rocha Francisco Airton Castro.(2007).Analgesic activity of a polysaccharide in experimental osteoarthritis in rats..Clinical rheumatology (8), 1312-9.

[12] Yanan Gao, Mingkun Guo, Jiaqi Chen, Yue Sun, Mingxing Wang. (2024). A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice.. International journal of biological macromolecules (P1), 133976.

Downloads

Published

29-11-2024

Issue

Section

Articles

How to Cite

Zhang, J., Deng, Y., Chen, J., Zhai, Y., Wang, F., & Yuan, F. (2024). Effects of Radix Achyrandii Polysaccharide on Cartilage Morphology of Knee Osteoarthritis in Obese Mice Induced by High-fat Diet. Academic Journal of Science and Technology, 13(2), 74-77. https://doi.org/10.54097/64zd5m60