The Expression of PI3K/Akt Pathway and Related Apoptotic Proteins in Early Retinal Blast Injury in Mice

Authors

  • Bo Wang
  • Yingxin Chen

DOI:

https://doi.org/10.54097/ajst.v4i2.4194

Keywords:

Retinal blast injury; PI3K/Akt pathway, Retinal cell apoptosis, Retinal ganglion cells, BAX, Bcl-2.

Abstract

This research sought to dissect out the expression of the PI3K/Akt pathway- and retinal apoptosis-related proteins in response to early retinal blast injury in mice. Methods: C57BL/6 mice were randomly and equally arranged into a normal control and an experimental group. The control mice were fed conventionally, and the experimental mice were subjected to blast shock. After successful modeling, mice were assigned into a 6-h experimental group and a 48-h experimental group according to sampling time. The pathological changes of mouse retina, the number of retinal ganglion cells (RGCs)were assessed by hematoxylin-eosin staining, and the apoptosis of retinal tissue was measured by TUNEL assay. PI3K and Akt phosphorylation and the expression of Bax, Bcl-2, and Cytochrome C proteins were determined using Western blot analysis. Results: Relative to the control mice, the experimental mice had loose retinal structure, disordered cell arrangement, and decreased RGCs. The 48-h experimental group showed more obvious RGCs reduction than the 6-h experimental group. Moreover, the apoptosis of retinal cells was higher in the experimental mice than in the control mice, and the apoptosis positive cells diffused from the inner layer to the outer layer of the retina. In contrast to the control mice, PI3K and Akt phosphorylation and BAX, Bcl-2, and Cytochrome C protein expressions were potently elevated in the experimental mice, and they were higher in the 48-h experimental group than in the 6-h experimental group. BAX/ Bcl-2 expression was decreased in the experimental group relative to that in the control group. Conclusion: Conclusively, the PI3K/Akt pathway was involved in early retinal blast injury in mice, and its effect was related to the upregulation of PI3K and Akt phosphorylation and Bcl-2 expression.

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Published

04-01-2023

How to Cite

Wang, B., & Chen, Y. (2023). The Expression of PI3K/Akt Pathway and Related Apoptotic Proteins in Early Retinal Blast Injury in Mice. Academic Journal of Science and Technology, 4(2), 158–161. https://doi.org/10.54097/ajst.v4i2.4194

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Articles