Comparative Analysis of the Effects of Different Chromosome Preparation Methods on Nuclear Morphology and Chromosome Structure

Xiaohui Shi

doi:10.54097/0sx56c83

Authors

Xiaohui Shi

DOI:

https://doi.org/10.54097/0sx56c83

Keywords:

Chromosome Preparation Methods, Chromosome Structure, Microscopy Techniques, Meiotic Chromosomes, Biological Sample Preparation

Abstract

Preparation of high quality chromosome samples is critical in cytogenetic research for accurate karyotyping and understanding cell biology. This paper examines the effects of different chromosome preparation methods on nuclear morphology and chromosome structure. It reviews the importance of chromosomes in genetics and cell biology and emphasizes the importance of high quality samples in karyotyping. Factors affecting chromosome quality include culture medium, processing intensity, chemical composition of fixatives, preparation methods, temperature, humidity, and airflow control. Evaluation of chromosome quality includes mitotic index, abnormal karyotype detection rate, and preparation success rate. Fluorescence in situ hybridization (FISH) is emerging as a promising high-resolution cytogenetic detection method, particularly for the detection of chromosomal abnormalities. This study examines the effects of different preparation methods on nuclear morphology and chromosome structure, providing insights into cell biology.

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