Research progress on the isolation and purification of ligninase
DOI:
https://doi.org/10.54097/jceim.v10i1.5421Keywords:
Lignin, Fibres, LigninaseAbstract
Lignin is a natural polymer substance with a phenolic structure, widely present in woody plants, herbs, vascular plants, is the second largest natural polymer material in nature after cellulose in quantity, and is the only aromatic compound that can be obtained from renewable resources in industry. Lignin is widely used in building materials industry, petroleum industry, light industry, and agriculture. Lignin is also an important part of human dietary fibres (DF), which can change the activity of microorganisms in the intestinal system, reduce the content of cholesterol and blood sugar in the blood, and have the effect of preventing cardiovascular disease; Lignin also has antioxidant activity and inhibition of cancer cell activity. As a natural antioxidant active substance, the anti-free radical activity of lignin in wood and crop straw has been extensively studied, while lignin in dietary fiber such as vegetables and fruits has not been studied much Extracting lignin from carrots and studying its structure and antioxidant activity is expected to obtain a natural, non-toxic side effect antioxidant active substance, which has a wide application prospect in the food industry, cosmetics, health products and other aspects.
References
Kumar M, Mishra A, Singh SS, et al. Expression and characterization of novel laccase gene from Pandoraea sp. ISTKB and its application [J]. International Journal of Biological Macromolecules, 2018.
Wang T N, Zhao M. A simple strategy for extracellular production of CotA laccase in Escherichia coli and decolorization of simulated textile effluent by recombinant laccase [J]. Applied Microbiology and Biotechnology, 2017, 101(2):685-696.
Hildén K, Mäkelä M R, Lundell T, Kuuskeri J, Chernykh A, Golovleva L, Archer, D B, Hatakka A. Heterologous expression and structural characterization of two low pH laccases from a biopulping white-rot fungus Physisporinus rivulosus [J]. Applied Microbiology & Biotechnology, 2013, 97(4):1589-1599.
Zheng M , Chi Y , Yi H , et al. Decolorization of Alizarin Red and other synthetic dyes by a recombinant laccase from Pichia pastoris [J]. Biotechnology Letters, 2014, 36(1):39-45.
Campos P A, Levin L N, Wirth S A. Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463 [J]. Process Biochemistry, 2016, 51(7):895-903.
Asses N, Ayed L, Hkiri N, Hamdi M. Congo Red Decolorization and Detoxification by Aspergillus niger: Removal Mechanisms and Dye Degradation Pathway [J]. BioMed Research International, 2018:1-9.
Mota T R, Kato C G, Peralta R A, Bracht A, Morais G R, Baesso M L, Peralta R M. Decolourization of Congo Red by Ganoderma lucidum Laccase: Evaluation of Degradation Products and Toxicity [J]. Water Air & Soil Pollution, 2015, 226(10):1-11.
Wang N, Chu Y, Wu F, et al. Decolorization and Degradation of Congo red by a newly isolated white rot fungus Ceriporia lacerate from decayed mulberry branches[J]. International Biodeterioration & Biodegradation, 2017, 117:236-244.
Zheng F, Cui B K, Wu X J, et al. Immobilization of laccase onto chitosan beads to enhance its capability to degrade synthetic dyes [J]. International Biodeterioration & Biodegradation, 2016, 110:69-78.
Madzak C, Mimmi M C, Caminade E, et al. Shifting the optimal pH of activity for a laccase from the fungus Trametes versicolor by structure-based mutagenesis [J]. Protein Engineering Design and Selection, 2006, 19(2):77-84.
Xu F, Palmer A E, Yaver D S, et al. Targeted Mutations in a Trametes villosa Laccase: AXIAL PERTURBATIONS OF THE T1 COPPER [J]. Journal of Biological Chemistry, 1999, 274(18):12372-12375.
Sun J, Peng R H, Xiong A S, Tian Y, Zhao W, Xu H, Liu D T, Chen J M, Yan Q H. Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris [J]. Molecular biology reports, 2012, 39(4):3807-3814.
Ma X, Liu L, Li Q, Liu Y, Yi L, Ma L, Zhai C. High-level expression of a bacterial laccase, CueO from Escherichia coli K12 in Pichia pastoris GS115 and its application on the decolorization of synthetic dyes [J]. Enzyme Microbial Technology, 2017, 103: 34–41.
Downloads
Published
Issue
Section
How to Cite
