Fiber Photometry for Measuring Neuronal Activities in the Subcortical Regions of the Central Auditory Pathway

Haoyang Yu

doi:10.54097/9790jt64

Authors

Haoyang Yu

DOI:

https://doi.org/10.54097/9790jt64

Keywords:

Fiber Photometry, Inferior Colliculus, Medial Geniculate Body, Genetically Encoded Calcium Indicators (GECIs), Internal Validity

Abstract

Fiber photometry has become a prominent tool for measuring population-level neuronal activity in the central auditory system (CANS). Yet, its application in subcortical regions, such as the inferior colliculus (IC) and medial geniculate body (MGB), remains underexplored. This review critically examines the limitations of in vivo fiber photometry in studying these regions, focusing on three key constraints: (1) low temporal resolution, which obscures millisecond-scale auditory responses; (2) lack of cell-type specificity, conflating excitatory and inhibitory signals; and (3) susceptibility to confounding variables (e.g., motion artifacts, GECI leakage). By synthesizing empirical studies from the IC and MGB, this review highlights how these limitations compromise data fidelity, particularly in the IC, where electrophysiology dominates due to its precision and accuracy. In the MGB, fiber photometry struggles with subregion-specific signal isolation and directional ambiguity in thalamocortical circuits. This review proposes that integrating fiber photometry with complementary techniques (e.g., optogenetics, electrophysiology) could mitigate these weaknesses. This review underscores the need for methodological innovations to enhance fiber photometry's utility in auditory neuroscience while clarifying its current constraints.

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References

[1] Simpson, E. H., Akam, T., Patriarchi, T., Blanco-Pozo, M., Burgeno, L. M., Mohebi, A., Cragg, S. J., & Walton, M. E. (2024). Lights, fiber, action! A primer on in vivo fiber photometry. Neuron, 112(5), 718–739. https://doi.org/10. 1016/j.neuron.2023.11.016.

[2] Driscoll, M. E., & Tadi, P. (2023). Neuroanatomy, Inferior Colliculus. In StatPearls [Internet]. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/sites/books/NBK554468/.

[3] Mei, H.-X., Cheng, L., & Chen, Q.-C. (2013). Neural interactions in unilateral colliculus and between bilateral colliculi modulate auditory signal processing. Frontiers in Neural Circuits, 7, 68. https://doi.org/10. 3389/ fncir. 2013. 00068.

[4] Sitek, K. R., Calabrese, E., Johnson, G. A., Ghosh, S. S., & Chandrasekaran, B. (2022). Structural Connectivity of Human Inferior Colliculus Subdivisions Using in vivo and post-mortem Diffusion MRI Tractography. Frontiers in Neuroscience, 16, 751595. https://doi.org/ 10.3389/ fnins. 2022. 751595.

[5] Jen, P. H., Chen, Q. C., & Sun, X. D. (1998). Corticofugal regulation of auditory sensitivity in the bat inferior colliculus. Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology, 183(6), 683–697. https://doi.org/10. 1007/s003590050291.

[6] Merchán, M., Aguilar, L. A., Lopez-Poveda, E. A., & Malmierca, M. S. (2005). The inferior colliculus of the rat: Quantitative immunocytochemical study of GABA and glycine. Neuroscience, 136(3), 907–925. https://doi.org/10. 1016/j. neuroscience. 2004.12.030.

[7] Pollak, G. D. (2013). The Dominant Role of Inhibition in Creating Response Selectivities for Communication Calls in the Brainstem Auditory System. Hearing Research, 305. https://doi.org/10.1016/j.heares.2013.03.001.

[8] Oberle, H. M., Ford, A. N., Dileepkumar, D., Czarny, J., & Apostolides, P. F. (2022). Synaptic mechanisms of top-down control in the non-lemniscal inferior colliculus. eLife, 10, e72730. https://doi.org/10.7554/eLife.72730.

[9] Lumani, A., & Zhang, H. (2010). Responses of neurons in the rat's dorsal cortex of the inferior colliculus to monaural tone bursts. Brain Research, 1351, 115–129. https://doi.org/10. 1016/j. brainres.2010.06.066.

[10] Valdés-Baizabal, C., Casado-Román, L., Bartlett, E. L., & Malmierca, M. S. (2021). In vivo whole-cell recordings of stimulus-specific adaptation in the inferior colliculus. Hearing Research, 399, 107978. https://doi.org/10.1016/j. heares. 2020. 107978.

[11] Suma Chinta, & Pluta, S. R. (2025). Whisking and locomotion are jointly represented in superior colliculus neurons. PLoS Biology, 23(4), e3003087–e3003087. https://doi.org/10.1371/ journal. pbio.3003087.

[12] Hu, F., & Dan, Y. (2022). An inferior-superior colliculus circuit controls auditory cue-directed visual spatial attention. Neuron, 110(1), 109-119.e3. https://doi.org/10.1016/j. neuron. 2021.10.004.

[13] Hormigo, S., e Horta Junior, J. de A. de C., Gómez-Nieto, R., & López García, D. E. (2012). The selective neurotoxin DSP-4 impairs the noradrenergic projections from the locus coeruleus to the inferior colliculus in rats. Frontiers in Neural Circuits, 6. https://doi.org/10.3389/fncir.2012.00041.

[14] Blackwell, J. M., Lesicko, A. M., Rao, W., De Biasi, M., & Geffen, M. N. (n.d.). Auditory cortex shapes sound responses in the inferior colliculus. eLife, 9, e51890. https://doi.org/10. 7554/ eLife.51890.

[15] Neher, E., & Sakaba, T. (2008). Multiple roles of calcium ions in the regulation of neurotransmitter release. Neuron, 59(6), 861-872. https://doi.org/10.1016/j.neuron.2008.09.004.

[16] Klug, A., Park, T. J., & Pollak, G. D. (1995). Glycine and GABA influence binaural processing in the inferior colliculus of the mustache bat. Journal of Neurophysiology, 74(4), 1701–1713. https://doi.org/10.1152/jn.1995.74.4.1701.

[17] Gittelman, J. X., Wang, L., Colburn, S. S., & Pollak, G. D. (2012). Inhibition shapes response selectivity in the inferior colliculus by gain modulation. Frontiers in Neural Circuits, 6. https://doi.org/10.3389/fncir.2012.00067.

[18] Pollak, G. D., Xie, R., Gittelman, J. X., Andoni, S., & Li, N. (2011). The dominance of inhibition in the inferior colliculus. Hearing Research, 274(1), 27–39. https://doi.org/ 10.1016/j. heares. 2010.05.010.

[19] Zhou, S., Zhu, Y., Du, A., Niu, S., Du, Y., Yang, Y., Chen, W., Du, S., Sun, L., Liu, Y., Wu, H., Lou, H., Li, X.-M., Duan, S., & Yang, H. (2025). A midbrain circuit mechanism for noise-induced negative valence coding. Nature Communications, 16(1), 4610. https://doi.org/10.1038/s41467-025-59956-z.

[20] Parker, D. (2022). Neurobiological reduction: From cellular explanations of behavior to interventions. Frontiers in Psychology, 13. https://doi.org/10.3389/fpsyg.2022.987101.

[21] Rees, A. (2009). Medial Geniculate Body. In M. D. Binder, N. Hirokawa, & U. Windhorst (Eds.), Encyclopedia of Neuroscience (pp. 2275–2279). Springer. https://doi.org/10. 1007/978-3-540-29678-2_3364.

[22] Bartlett, E. L. (2013). The organization and physiology of the auditory thalamus and its role in processing acoustic features important for speech perception. Brain and Language, 126(1), 29–48. https://doi.org/10.1016/j.bandl.2013.03.003.

[23] Anderson, L. A., & Linden, J. F. (2011). Physiological differences between histologically defined subdivisions in the mouse auditory thalamus. Hearing Research, 274(1), 48–60. https://doi.org/10.1016/j.heares.2010.12.016.

[24] Bartlett, E. L., & Smith, P. H. (1999). Anatomic, Intrinsic, and Synaptic Properties of Dorsal and Ventral Division Neurons in Rat Medial Geniculate Body. Journal of Neurophysiology, 81(5), 1999–2016. https://doi.org/10.1152/jn.1999.81.5.1999.

[25] Chen, T.-W., Wardill, T. J., Sun, Y., Pulver, S. R., Renninger, S. L., Baohan, A., Schreiter, E. R., Kerr, R. A., Orger, M. B., Jayaraman, V., Looger, L. L., Svoboda, K., & Kim, D. S. (2013). Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature, 499(7458), 295–300. https://doi.org/ 10.1038/nature12354.

[26] Reiff, D. F., Ihring, A., Guerrero, G., Isacoff, E. Y., Joesch, M., Nakai, J., & Borst, A. (2005). In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies. The Journal of Neuroscience, 25(19), 4766–4778. https://doi.org/ 10. 1523/JNEUROSCI.4900-04.2005.

[27] Winer, J. A. (1992). The Functional Architecture of the Medial Geniculate Body and the Primary Auditory Cortex. In D. B. Webster, A. N. Popper, & R. R. Fay (Eds.), The Mammalian Auditory Pathway: Neuroanatomy (pp. 222–409). Springer. https://doi.org/10.1007/978-1-4612-4416-5_6.

[28] Morest, D. K. (1964). The neuronal architecture of the medial geniculate body of the cat. Journal of Anatomy, 98(Pt 4), 611-630.1.

[29] Mihai, P. G., Moerel, M., Martino, F. de, Trampel, R., Kiebel, S., & Kriegstein, K. von. (2019). Modulation of tonotopic ventral medial geniculate body is behaviorally relevant for speech recognition. ELife, 8. https://doi.org/10. 7554/ elife. 44837.

[30] Kriegstein, K. von, Patterson, R. D., & Griffiths, T. D. (2008). Task-Dependent Modulation of Medial Geniculate Body Is Behaviorally Relevant for Speech Recognition. Current Biology, 18(23), 1855–1859. https://doi.org/10.1016/j.cub. 2008.10.052.

[31] Guo, W., Clause, A. R., Barth-Maron, A., & Polley, D. B. (2017). A corticothalamic circuit for dynamic switching between feature detection and discrimination. Neuron, 95(1), 180-194.e5. https://doi.org/10.1016/j.neuron.2017.05.019.

[32] Homma, N. Y., Happel, M. F. K., Nodal, F. R., Ohl, F. W., King, A. J., & Bajo, V. M. (2017). A Role for Auditory Corticothalamic Feedback in the Perception of Complex Sounds. The Journal of Neuroscience, 37(25), 6149–6161. https://doi.org/10.1523/JNEUROSCI.0397-17.2017.

[33] Gilad, A., Maor, I., & Mizrahi, A. (2020). Learning-related population dynamics in the auditory thalamus. eLife, 9, e56307. https://doi.org/10.7554/eLife.56307.

[34] Li, J., Liao, X., Zhang, J., Wang, M., Yang, N., Zhang, J., Lv, G., Li, H., Lu, J., Ding, R., Li, X., Guang, Y., Yang, Z., Qin, H., Jin, W., Zhang, K., He, C., Jia, H., Zeng, S., … Chen, X. (2017). Primary Auditory Cortex is Required for Anticipatory Motor Response. Cerebral Cortex, 27(6), 3254–3271. https:// doi. org/10.1093/cercor/bhx079.

[35] Antunes, F. M., & Malmierca, M. S. (2021). Corticothalamic Pathways in Auditory Processing: Recent Advances and Insights From Other Sensory Systems. Frontiers in Neural Circuits, 15. https://doi.org/10.3389/fncir.2021.721186.