The Evolutionary Process of Acid Adaptation and Deep Transcriptional Analysis in Bacillus subtilis 168
DOI:
https://doi.org/10.54097/yqdfgp26Abstract
This study is focused on investigating the variations in gene expression of Bacillus subtilis 168 following acid adaptation evolution. Through simulating prolonged cultivation in a natural acidic setting, we have observed the occurrence of adaptive evolution within the strain and conducted an extensive analysis of gene expression in the evolved strain using high-throughput transcriptomics technology. The findings from this research shed light on the fundamental molecular mechanisms employed by Bacillus subtilis to adapt to acidic environments, thereby providing crucial scientific evidence for comprehending its survival strategies and potential applications in acidic settings.
Downloads
References
AESN, EAA, SAS, et al. Antibacterial and anticancer activity of ε-poly-L-lysine (ε-PL) produced by a marine Bacillus subtilis sp.[J]. Journal of Basic Microbiology, 2012, 52(5).
Westers L, Westers H, Quax W J. Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism[J]. Biochim Biophys Acta, 2004, 1694(1-3): 299-310.
Yonghua S. Expression and Character of Three Plasmids from Staphylococcus aureus Strain 307 in Bacillus sttbtilis Strains Ki-2 and 168[J]. Acta Genetica Sinica, 1983.
Koo B M, Kritikos G, Farelli J D, et al. Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis[J]. Cell Syst, 2017, 4(3): 291-305.
Zhao X Y, Xu J Y, Tan M, et al. Construction of a plasmid interspecific transfer system in Bacillus species with the counter-selectable marker mazF[J]. Journal of Industrial Microbiology & Biotechnology, 2018.
Sandberg T E, Salazar M J, Weng L L, et al. The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology[J]. Metabolic Engineering, 2019, 56.
Wu Y, Jameel A, Xing Xh, et al. Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution.[J]. Trends in biotechnology, 2021.
Gibson B, Dahabieh M, Krogerus K, et al. Adaptive Laboratory Evolution of Ale and Lager Yeasts for Improved Brewing Efficiency and Beer Quality[J]. Annual Review of Food Science and Technology, 2020, 11(1).
Bachmann H, Pronk J T, Kleerebezem M, et al. Evolutionary engineering to enhance starter culture performance in food fermentations[J]. Current Opinion in Biotechnology, 2015, 32.
Royce L A, Yoon J M, Chen Y, et al. Evolution for exogenous octanoic acid tolerance improves carboxylic acid production and membrane integrity[J]. Metabolic Engineering, 2015, 29: 180-188.
R D, R S, C W, et al. Adaptation of Saccharomyces cerevisiae to saline stress through laboratory evolution.[J]. Journal of Evolutionary Biology, 2011, 24(5).
Dhar, Sgesser, Yuan, et al. Adaptation of Saccharomyces cerevisiae to saline stress through laboratory evolution[J].
Xu X, Williams T C, Divne C, et al. Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance[J]. Biotechnology for Biofuels, 2019, 12(1).
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Academic Journal of Science and Technology
This work is licensed under a Creative Commons Attribution 4.0 International License.
