Isolation and Identification of the Pathogen Causing Polygonatum Leaf Spot and Screening of Botanical Fungicides

Shan Liu; Yu Huang; Hongbo Zhang; Dan  Yang

doi:10.54097/3471gv98

Authors

Shan Liu
Yu Huang
Hongbo Zhang
Dan Yang

DOI:

https://doi.org/10.54097/3471gv98

Keywords:

Polygonatum, Leaf Spot Disease, Fusarium, Plant Extracts, Antibacterial Activity

Abstract

In order to investigate the incidence of Polygonatum leaf spot disease in Enshi City, Hubei Province, identify its pathogenic fungi, and screen plant extracts with effective antibacterial activity against the pathogen, this study systematically investigated the occurrence of leaf spot disease in the Polygonatum cultivation base of Laifeng County, Enshi City. Leaves of Polygonatum seedlings with typical leaf spot symptoms were collected for pathogen isolation. The pathogen was identified through morphological characteristics and molecular analysis, and its pathogenicity was verified by seedling inoculation. The mycelial growth rate method was employed to evaluate the antibacterial activity of nine plant extracts. Results showed that a highly pathogenic strain FR-1 was isolated and identified as Fusarium incarnatum. Among the nine plant extracts, only Coptis chinensis water extract and Berberis julianae water extract exhibited significant inhibitory effects. The 50 mg/mL Coptis chinensis water extract demonstrated 82.43% inhibition rate against F. incarnatum with EC50 of 6.249 mg/mL, and 77.50% spore inhibition rate with EC50 of 8.236 mg/mL. In conclusion, Coptis chinensis shows potential for development as a botanical fungicide.

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