Progress in Genetic Engineering Research of Silkworms
DOI:
https://doi.org/10.54097/7w654n61Keywords:
Silkworm, Genome Editing, Silk, Disease-resistant, GenomicsAbstract
The genetic breeding of silkworms has established a sound mechanism for silkworm breeding and preservation in previous research, which has steadily promoted the industrial development of silkworms. However, with the continuous advancement of technology, traditional genetic resource development has gradually encountered development bottlenecks and has reached saturation. Therefore, turning to new technological means has become an urgent task. In recent years, the rapid development of molecular biology, genomics, genetic modification, and genome editing technologies has opened up new opportunities for the genetic breeding of silkworms. The application potential of these modern technologies not only promotes the transformation and upgrading of silkworm genetic breeding, but also points out the direction for its future development. In summary, future research and applications in silkworm genetic breeding will focus more on transgenic and genome editing technologies, which will provide new opportunities and ideas for the improvement of varieties and the cultivation of new silkworm breeds.
Downloads
References
[1] Ran Zhang , Your daughter Wang, Y. Bao, J. Zhao, N. Li. Research status and biosafety evaluation of transgenic animal applications. Bioindustry Technology, 2010(3): 48-59.
[2] ZHANG R, WANG Y Y, BAO Y H, ZHAO J, LI N. Current status and biosafety evaluation of transgenic animals. Biotechnology and Business, 2010(3): 48-59. (in Chinese). Biotechnology and Business, 2010(3): 48-59.
[3] Adrianos SL, Teulé F, Hinman MB, et al. Nephila clavipes Flagelliform silk-like GGX motifs contribute to extensibility and spacer motifs contribute to strength in synthetic spider silk fibers. biomacromolecules. 2013;14(6):1751-1760.
[4] Asadpour S, Kargozar S, Moradi L, Ai A, Nosrati H, Ai J. Natural biomacromolecule based composite scaffolds from silk fibroin, gelatin and chitosan toward tissue engineering applications. Int J Biol Macromol. 2020;154:1285-1294.
[5] Song J, Li Z, Zhou L, et al. FOXO-regulated OSER1 reduces oxidative stress and extends lifespan in multiple species. Nat Commun. 2024;15(1):7144.
[6] Dong Z, Huang L, Dong F, et al. Establishment of a baculovirus-inducible CRISPR/Cas9 system for antiviral research in transgenic silkworms. Appl Microbiol Biotechnol. 2018;102(21):9255-9265.
[7] Dong Z, Hu Z, Qin Q, et al. CRISPR/Cas9-mediated disruption of the immediate early-0 and 2 as a therapeutic approach to Bombyx mori nucleopolyhedrovirus in transgenic silkworm. Insect Mol Biol. 2019;28(1):112-122.
[8] Eckermann KN, Ahmed HMM, KaramiNejadRanjbar M, et al. Hyperactive piggyBac transposase improves transformation efficiency in diverse insect species . Insect Biochem Mol Biol. 2018;98:16-24.
[9] Fujinaga D, Kohmura Y, Okamoto N, Kataoka H, Mizoguchi A. Insulin-like growth factor (IGF)-like peptide and 20-hydroxyecdysone regulate the growth and development of the male genital disk through different mechanisms in the silkmoth, Bombyx mori. Insect Biochem Mol Biol. 2017;87:35-44.
[10] Gholipourmalekabadi M, Sapru S, Samadikuchaksaraei A, Reis RL, Kaplan DL, Kundu SC. Silk fibroin for skin injury repair: where do things stand? Adv Drug Deliv Rev. 2020; 153:28-53.
[11] Golicz AA, Bayer PE, Bhalla PL, Batley J, Edwards D. Pangenomics Comes of Age: From Bacteria to Plant and Animal Applications. Trends Genet. 2020;36(2). 132-145.
[12] Guha TK, Calos MP. Nucleofection of phiC31 Integrase Protein Mediates Sequence-Specific Genomic Integration in Human Cells. j Mol Biol. 2020;432(13). 3950-3955.
[13] Gong Z, Wu T, Chen X, Guo J, Zhang Y, Li Y. Upconversion Nanoparticle Decorated Spider Silks as Single-Cell Thermometers. Nano Lett. 2021;21(3):1469- 1476.
[14] Han MJ, Luo C, Hu H, et al. Multiple independent origins of the female W chromosome in moths and butterflies. Sci Adv. 2024;10(25): eadm9851.
[15] He Q, Zhang Y. Kr-h1, a Cornerstone Gene in Insect Life History. Front Physiol. 2022; 13:905441.
[16] He X, Gu F. Sheng Wu Gong Cheng Xue Bao. 2017;33 (10): 1757-1775.
[17] Hu W, Jia A, Ma S, et al. A molecular atlas reveals the tri-sectional spinning mechanism of spider dragline silk. Nat Commun. 2023;14(1):837.
[18] Huang W, Ling S, Li C, Omenetto FG, Kaplan DL. Silkworm silk-based materials and devices generated using bio-nanotechnology. Chem Soc Rev. 2018;47(17). 6486-6504.
[19] Imamura M, Nakahara Y, Kanda T, Tamura T, Taniai K. A transgenic silkworm expressing the immune-inducible cecropin B-GFP reporter gene. insect Biochem Mol Biol. 2006;36(5):429-434.
[20] Khosropanah MH, Vaghasloo MA, Shakibaei M, et al. Biomedical applications of silkworm (Bombyx Mori) proteins in regenerative medicine (a narrative review). Tissue Eng Regen Med. 2022;16(2):91-109.
[21] Kluge JA, Rabotyagova O, Leisk GG, Kaplan DL. Spider silks and their applications. Trends Biotechnol. 2008;26(5):244-251.
[22] Li K, Dong Z, Pan M. Common strategies in silkworm disease resistance breeding research. Pest Manag Sci. 2023;79(7): 2287-2298.
[23] Li Z, You L, Zeng B, et al. Ectopic expression of ecdysone oxidase impairs tissue degeneration in Bombyx mori. Proc Biol Sci. 2015;282(1809):20150513.
[24] Li Z, Qian W, Song W, et al. A salivary gland-secreted peptide regulates insect systemic growth. Cell Rep. 2022;38(8):110397.
[25] Li Z, You L, Zhang Q, Yu Y, Tan A. A Targeted In-Fusion Expression System for Recombinant Protein Production in Bombyx mori. Front Genet. 2022; 12:816075.
[26] Ling L, Ge X, Li Z, et al. MiR-2 family targets awd and fng to regulate wing morphogenesis in Bombyx mori. RNA Biol. 2015;12(7):742-748.
[27] Liu S, Li K, Gao Y, et al. Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila. Proc Natl Acad Sci U S A. 2018;115(1):139-144.
[28] Long D, Lu W, Hao Z, Xiang Z, Zhao A. Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system. transgenic Res. 2016;25(6):795-811.
[29] Ma Y, Li Q, Tang Y, et al. The architecture of silk-secreting organs during the final larval stage of silkworms revealed by single-nucleus and spatial transcriptomics. Cell Rep. 2024;43(7):114460.
[30] Pacesa M, Pelea O, Jinek M. Past, present, and future of CRISPR genome editing technologies. cell. 2024;187(5):1076-1100.
[31] Parent LR, Onofrei D, Xu D, et al. Hierarchical spidroin micellar nanoparticles as the fundamental precursors of spider silks. Proc Natl Acad Sci U S A. 2018;115(45):11507-11512.
[32] Park YN, Masison D, Eisenberg E, Greene LE. Application of the FLP/FRT system for conditional gene deletion in yeast Saccharomyces cerevisiae. Yeast. 2011;28(9):673-681.
[33] Sadat A, Biswas T, Cardoso M H, et al. Silkworm pupae as a future food with nutritional and medicinal benefits. Curr Opin Food Sci. 2022, 44, 100818.
[34] Shao Z, Vollrath F. Surprising strength of silkworm silk. Nature. 2002;418(6899):741.
[35] Song K, Wang Y, Dong W, et al. Decoding silkworm spinning programmed by pH and metal ions. Sci Bull (Beijing). 2024;69(6):792-802.
[36] Sudmant PH, Rausch T, Gardner EJ, et al. An integrated map of structural variation in 2,504 human genomes. nature. 2015;526(7571):75-81.
[37] Tan A, Fu G, Jin L, et al. Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori. Proc Natl Acad Sci U S A. 2013;. 110(17):6766-6770.
[38] Wang RJ, Chen K, Xing LS, Lin Z, Zou Z, Lu Z. Reactive oxygen species and antimicrobial peptides are sequentially produced in silkworm midgut in response to bacterial infection. Dev Comp Immunol. 2020; 110:103720.
[39] Wang C, Wang K, Kou Y. Genome editing creates disease-resistant crops without yield penalties. Trends Plant Sci. 2024;29(2):114-116.
[40] Wang C, Yu B, Meng X, et al. Microsporidian Nosema bombycis hijacks host vitellogenin and restructures ovariole cells for transovarial transmission. PLoS Pathog. 2023;19(12): e1011859.
[41] Wang Y, Ren J, Ye C, Pei Y, Ling S. Thermochromic Silks for Temperature Management and Dynamic Textile Displays. Nanomicro Lett. 2021;13(1):72.
[42] Xia Q, Guo Y, Zhang Z, et al. Complete resequencing of 40 genomes reveals domestication events and genes in silkworm (Bombyx). Science. 2009;326(5951):433-436.
[43] Xiang H, Liu X, Li M, et al. The evolutionary road from wild moth to domestic silkworm. Nat Ecol Evol. 2018;2(8):1268-1279.
[44] Xu J, Dong Q, Yu Y, et al. Mass spider silk production through targeted gene replacement in Bombyx mori. Proc Natl Acad Sci U S A. 2018;115(35):8757-8762.
[45] Yang Y, Zhao T, Li Z, et al. Histone H3K27 methylation-mediated repression of Hairy regulates insect developmental transition by modulating ecdysone biosynthesis. Proc Natl Acad Sci U S A. 2021;118(35):e2101442118.
[46] Yu Y, Chen K, Wang J, et al. Custom-designed, mass silk production in genetically engineered silkworms. PNAS Nexus. 2024;3(4):pgae128.
[47] Zeng B, Zhan S, Wang Y, et al. Expansion of CRISPR targeting sites in Bombyx mori. Insect Biochem Mol Biol. 2016;72:31-40.
[48] Yuan D, Zhang X, Yang Y, et al. Schlank orchestrates insect developmental transition by switching H3K27 acetylation to trimethylation in the prothoracic gland. Proc Natl Acad Sci U S A. 2024;121(35):e2401861121.
[49] Zhang T, Song W, Li Z, et al. Krüppel homolog 1 represses insect ecdysone biosynthesis by directly inhibiting the transcription of steroidogenic enzymes. Proc Natl Acad Sci U S A. 2018;115(15):3960-3965.
[50] Zhang Z, Liu X, Shiotsuki T, et al. Depletion of juvenile hormone esterase extends larval growth in Bombyx mori. Insect Biochem Mol Biol. 2017; 81:72-79.
[51] Zhao Y, Guan J, Wu SJ. Highly Stretchable and Tough Physical Silk Fibroin-Based Double Network Hydrogels. Macromol Rapid Commun. 2019;40(23):e1900389.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
