Preparation and Functional Verification of LAPTM4B Monoclonal Antibody

Shenli Huang; Xincai Han; Mi Zhang

doi:10.54097/8j8az896

Authors

Shenli Huang
Xincai Han
Mi Zhang

DOI:

https://doi.org/10.54097/8j8az896

Keywords:

LAPTM4B, Monoclonal Antibody, Immunoblotting, Immunohistochemistry

Abstract

Lysosomal-associated transmembrane protein 4 beta (LAPTM4B) is a lysosomal transmembrane protein that is highly expressed in a variety of malignant tumors. In order to prepare LAPTM4B monoclonal antibody with strong specificity and high sensitivity, BALB / c mice were immunized with the target protein, and the spleen of the mice was fused with SP2 / 0. The hybridoma cells that can stably secrete antibodies were screened by indirect Elisa and Western blot. Ascites was prepared and purified to obtain antibodies. The specificity, sensitivity and scope of application of the antibody were verified by Western blot and IHC. Results: Thirty-six hybridoma cells stably secreting antibodies were successfully screened, and nine monoclonal antibodies were obtained. One of them had good characteristics in Western blot and IHC, which laid a foundation for further study of the biological characteristics of LAPTM4 B and the development of blocking antibodies for tumor therapy.

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