Comparative Study of Black Daqu Blocks and Normal Daqu in Strong-Flavor Daqu

Weihao Zhang; Guangbin Ye

doi:10.54097/sqaeh742

Authors

Weihao Zhang
Guangbin Ye

DOI:

https://doi.org/10.54097/sqaeh742

Keywords:

Strong-flavor Daqu, Black Daqu Blocks, Microbial Communities, Physicochemical Properties

Abstract

Due to the heterogeneity of solid-state fermentation, black Daqu blocks often form in the cross-sections of strong-flavor Daqu. These black blocks exhibit microbial communities and physicochemical properties significantly different from normal Daqu. This study aims to investigate the impact of black blocks on the quality of Daqu by comparing the microbial diversity, enzymatic properties, and physicochemical indicators of black blocks and normal Daqu, and by conducting correlation analyses to uncover the underlying mechanisms. The results showed that the microbial diversity in normal Daqu was higher than that in black blocks. Normal Daqu was rich in yeast species, including Dipodascaceae sp., Candida tropicalis, and Wickerhamomyces anomalus, as well as bacteria dominated by lactic acid bacteria and Bacillus species, such as Enterococcus faecalis, Weissella confusa, Bacillus licheniformis, and Bacillus amyloliquefaciens. Normal Daqu exhibited superior acidity and fermentation capacity. In contrast, the fungal community in black blocks was predominantly composed of Thermomyces lanuginosus, Wallemia mellicola, and Aspergillus chevalieri. while the bacterial community was dominated by Staphylococcus gallinarum. Black blocks demonstrated stronger saccharification and liquefaction abilities. Correlation analysis indicated that the primary microorganisms in normal Daqu were significantly associated with acidity and fermentation capacity. whereas the dominant microbial groups in black blocks were correlated with moisture, saccharification, and liquefaction abilities. This study highlights the quality differences between black blocks and normal Daqu in strong-flavor Daqu, providing theoretical insights for optimizing Daqu production.

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