Study on Expression Differences of SMAD3 Gene in Different Tissues of Grouper and Its Correlation with Muscle Fiber Development
DOI:
https://doi.org/10.54097/34rhbt71Keywords:
Grouper, SMAD3 Gene, Tissue-specific Expression, Muscle Fiber DevelopmentAbstract
This study aims to explore the expression pattern of the SMAD3 gene in different tissues of grouper and its potential correlation with muscle fiber development. Using real-time fluorescent quantitative PCR, the relative expression levels of the SMAD3 gene were detected in various tissues of grouper, including the heart, liver, spleen, kidney, and muscle. The results showed that the SMAD3 gene exhibited significant expression differences among different tissues, with relatively high expression in muscle tissue. Further histological and correlation analyses were conducted to investigate the relationship between SMAD3 gene expression and developmental indicators such as muscle fiber diameter and density. The findings provide an important basis for understanding the molecular mechanism of grouper muscle development and offer theoretical support for genetic improvement and aquaculture production of grouper.
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