Construction of an Exogenous Green Fluorescent Protein (GFP) Gene Expression Vector and its Expression Verification in Tobacco Leaves

Abstract

This study employed genetic engineering techniques to design and construct a specific expression vector containing the GFP gene. Subsequently, the constructed vector was transformed into Agrobacterium GV3101 via Agrobacterium infection of tobacco leaves. Experimental results demonstrated that the constructed exogenous GFP gene vector successfully achieved transient expression in tobacco leaves, which emitted distinct green fluorescence. This study not only validated the effectiveness of the gene expression vector construction but also provided crucial practical experience for further investigating gene functions within plants and advancing plant genetic improvement efforts.