The Plant-Endophyte-Synthetic Biology Trinity: A Next-Generation Biorefinery Platform for Lignocellulose Valorization and Precision Polysaccharide Biomanufacturing
DOI:
https://doi.org/10.54097/4zg3f345Keywords:
Lignocellulose, Polysaccharide, Synthetic Biology, Endophyte, Microbial Consortia, Consolidated Bioprocessing (CBP)Abstract
The establishment of a robust bioeconomy depends on sustainable lignocellulosic biomass valorization and efficient production of high-value functional polysaccharides. The current bio-manufacturing model faces significant hurdles, including high refined sugar costs and energy-intensive pre-treatment. This review proposes an innovative “plant endophytic fungi-synthetic biology” trinity strategy to address these challenges. We describe how engineered plant endophytic fungal systems act as programmable in situ bioreactors to promote the decomposition of lignocellulose through rational design and synthesis of microbial consortia (SynComs) and integrated biological treatment (CBP). At the same time, the framework utilizes a cutting-edge synthetic biology toolbox-cross-genome editing, metabolic engineering, and epigenetic regulation - to achieve precise biomanufacturing of structurally diverse polysaccharides through host metabolic reprogramming. We critically assess industrial bottlenecks, from consortium stability to structural-functional knowledge gaps, while outlining future frontiers such as artificial intelligence-driven rational design and the biological basis of automation. This three-in-one strategy represents a paradigm shift from resource-intensive extraction to intelligent biomanufacturing, providing a theoretical basis and practical roadmap for realizing the full potential of lignocellulose in modern biorefinery.
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