Differences in Gel Properties of Soy Protein Induced by Fermentation with Different Lactic Acid Bacteria and Comparative Analysis of Related Genomes
DOI:
https://doi.org/10.54097/gz6daq26Keywords:
Lactic acid bacteria; Soy protein gel properties; Genome comparison; Gene enrichment analysis.Abstract
Lactic acid bacteria (LAB) play a crucial role in the formation and functional property regulation of soy protein gels. However, significant differences exist in the effects of different strains on gel properties, and the underlying molecular-level differences have not been fully elucidated. In this study, eight LAB strains commonly used in food production, including Lactobacillus acidophilus, Lactobacillus plantarum, Lacticaseibacillus casei, Lacticaseibacillus paracasei, Lacticaseibacillus rhamnosus, Leuconostoc mesenteroides, Lactococcus lactis, and Streptococcus thermophilus, were selected to systematically evaluate five core texture indicators of fermented soy protein gels: hardness, springiness, cohesiveness, gumminess, and chewiness. Meanwhile, combined with the genome sequencing data of each strain, the differences in the effects of different strains on gel texture were compared, and functional enrichment analysis was performed on differentially expressed coding genes. The results showed that the eight LAB strains exhibited differences in the number of coding genes and unique genes in carbohydrate-related pathways, nucleotide metabolism pathways, lysine/arginine biosynthesis pathways, and ABC transporter pathways, and the induced gels also showed different texture properties. This study not only reveals the significant differences in texture properties of soy protein gels induced by different LAB strains but also further clarifies the core root factor regulating gel properties, namely the differences in strain coding genes. The purpose of this study is to provide a theoretical reference and key research entry point for the subsequent directional improvement of soy protein gel properties through precise regulation of strain genes.
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