Bioinformatic Analysis of the Soybean MYB Transcription Factor Family
DOI:
https://doi.org/10.54097/wxq0a220Keywords:
Soybean; MYB Transcription Factor; Bioinformatics analysis; Gene family; Domain.Abstract
Soybean (Glycine max) is an important grain and oil crop worldwide, and its yield and adaptability are regulated by multiple transcription factors. The MYB transcription factor family plays key roles in plant growth and development, secondary metabolism, and stress responses. In this study, 62 GmMYB genes were systematically identified based on soybean whole-genome data. Their conserved domains, conserved motifs, phylogenetic relationships, and three-dimensional protein structures were then analyzed. The results showed that GmMYB proteins could be divided into three subfamilies, namely 2R-MYB, 1R-MYB, and ZNF-1R-MYB, with 2R-MYB being the predominant type. Conserved motif analysis showed that Motifs 1-4 were the core motifs of R2R3-MYB proteins and were present in most members. Three-dimensional structure prediction indicated that all GmMYB proteins mainly adopted random-coil/beta-turn conformations, whereas alpha-helices accounted for 37%-40% and formed the structural framework for DNA-binding function. Some members differed in helical proportion and flexible regions, which may be related to their functional divergence. This study provides a theoretical basis for functional analysis of soybean MYB transcription factors and stress-resistance breeding.
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