MiR-133b Targets FSCN1 to Inhibit the Malignant Progression of Oral Squamous Cell Carcinoma
DOI:
https://doi.org/10.54097/8r85x529Keywords:
Head and Neck Squamous Cell Carcinoma, miR-133b, Transcriptome Data, Cell Invasion, MetastasisAbstract
Oral squamous cell carcinoma (OSCC) poses a significant challenge due to its aggressive nature and propensity for metastasis, leading to poor patient outcomes. Current treatment options for advanced-stage OSCC are limited, highlighting the urgent need for novel therapeutic approaches. In this context, microRNA-133b (miR-133b) has emerged as a promising candidate with tumor-suppressive properties across various cancer types, including OSCC. However, the specific mechanisms underlying its actions in OSCC have not been fully elucidated. To address this gap in knowledge, our study employed a multifaceted approach combining bioinformatics analysis of comprehensive datasets from TCGA and GEO databases with functional experiments in vitro. Our results unveiled miR-133b as a pivotal prognostic miRNA in OSCC, with overexpression significantly inhibiting the growth, migration, and invasion of OSCC cells. Through a series of molecular assays, we identified FSCN1 as a direct target of miR-133b,providing mechanistic insights into its tumor-suppressive role. Clinically, high levels of FSCN1 were associated with adverse clinicopathological features and poor survival outcomes in OSCC patients, underscoring its potential as a prognostic biomarker. Furthermore, gene set enrichment analysis revealed a link between FSCN1 overexpression and the unfolded protein response pathway, shedding light on potential therapeutic vulnerabilities in OSCC. In conclusion, our findings highlight the significance of the miR-133b/FSCN1 axis in suppressing OSCC progression, suggesting its promise as both a prognostic indicator and a target for novel therapeutic interventions. This study enhances our understanding of the complex molecular landscape of OSCC and paves the way for future clinical applications in improving patient outcomes.
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