Simultaneous Determination of Zearalenone and Aflatoxin B1 in Maize by High Performance Liquid Chromatography

Abstract

This experiment established a method for the simultaneous detection of Zearalenone (ZEN) and Aflatoxin B1 (AFB1) in corn using High-Performance Liquid Chromatography with fluorescence detector. The sample was extracted with acetonitrile: 0.2% formic acid water (volume ratio: 80:20) and passed through a Prime HLB 3cc purification column. Liquid phase detection conditions: The chromatographic column is Agilent C18 (150mm×4.6mm,5μm) The mobile phase is acetonitrile: water (volume ratio: 80:20). The retention time is qualitative and the external standard method is quantitative. The results showed that the correlation coefficients for ZEN 20～1000ng/mL and AFB1 1～50ng/mL were 0.9989 and 0.9976, respectively, and the detection limits were 5μg/kg and 0.8 μg/kg, with an average spiked recovery of 92.7% to 106.2%. This study combines the detection methods in the current two national standards into one, which can simultaneously achieve rapid and synchronous detection of ZEN and AFB1 in corn. The required extraction and detection time is reduced from 60 minutes to 30 minutes, which is 50% less than the original time. The detection results have high accuracy and good stability.