Young and Senescent Cells: Distinct Nuclear F-actin Patterns Upon Latrunculin B Induction

Dichun Huang

doi:10.54097/ijbls.v2i3.8644

Authors

Dichun Huang

DOI:

https://doi.org/10.54097/ijbls.v2i3.8644

Keywords:

Cellular Senescence, Actin Cytoskeleton, Latrunculin B, Nucleus, F-actin

Abstract

Both cellular senescence and cytoskeleton are involved in the formation of many diseases and cell signaling pathways. Although recent studies have shown that F-actin is involved in DNA damage repair, chromatin decompression, gene transcription regulation, and cell fate determination. But studies on F-actin and aging are still absence. It is unclear whether nuclear F-actin is present during cellular senescence. Here, by confocal optical sectioning and time-lapse imaging, we found acitn chrommobody-TagGFP2-NLS shows the beneficial on investigating senescent human fibroblast IMR-90 cells. To induce the nuclear F-actin assembly in single cell, we uesd Latrunculin B (latB) which a cytoplasmic F-actin polymerization inhibitor. It is currently unknown whether the nuclear F-actin cytoskeleton in young and senescent cells responds differently to latB treatment. Here, latB application induces distinct nuclear F-actin patterns and dynamics in young and senescent cells. Thus, after analyzing the results of actin dynamic we demonstrate a diverse effect of latB on the nuclear F-actin cytoskeleton in young and senescent cells.

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References

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