Gene Drive System Based on CRISPR/Cas9 in Mosquito Control for Preventing Malaria
DOI:
https://doi.org/10.54097/6sv5gk97Keywords:
Malaria, CRISPR/Cas9, Mosquito, Gene drive, Parasite.Abstract
Malaria is a disease transmitted by mosquitoes to humans and has symptoms varying from mild such as fever and headache to severe such as loss of consciousness, bleeding, or even death. Although there are a few other pathways to get infected with malaria, female mosquitoes belonging to the genus Anopheles infected by pathogen parasites belonging to Plasmodium have been considered as the predominant media of transmission, and elimination of this pathway could greatly assist in controlling the spread of malaria among populations. CRISPR/Cas9, as an emerging genome editing technology, functions by designed guide RNA (gRNA) for targeting sequence and Cas9 for cleaving targeted double-stranded DNA. Due to the great power of CRISPR/Cas9 in gene editing, it has significantly facilitated the study of mosquito control. This review describes the potential and applicable properties of the CRISPR/Cas9 system in restricting the spread of malaria via genetic screening of mosquitoes and gene-drive population suppression by modifying female mosquito fertility to reduce pathogen infection rate by targeting alleles from mosquitoes and pathogen to prohibit mosquito infections or retard the parasite development process. Furthermore, this review discusses the urgency that adjusting CRISPR/Cas9 technique to be affordable by more performers with low off-target effect. In conclusion, CRISPR/Cas9 system is expected to affirm the applicability of the technique in nature to restrict the spread of malaria among populations.
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References
World Health Organization. (2023). Fact sheet about malaria. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/malaria
Mayo Clinic. (2023). Malaria. Mayo Clinic. https://www.mayoclinic.org/diseases-conditions/malaria/symptoms-causes/syc-20351184
Hossain, M. A. (2021). CRISPR-Cas9: A fascinating journey from bacterial immune system to human gene editing. Progress in Molecular Biology and Translational Science, 63–83.
Redman, M., King, A., Watson, C., & King, D. (2016). What is CRISPR/cas9? Archives of Disease in Childhood - Education & Practice Edition, 101(4), 213–215.
Liu, W., Li, L., Jiang, J., Wu, M., & Lin, P. (2021). Applications and challenges of CRISPR-cas gene-editing to disease treatment in clinics. Precision Clinical Medicine, 4(3), 179–191.
Viswanatha, R., Mameli, E., Rodiger, J., Merckaert, P., Feitosa-Suntheimer, F., Colpitts, T. M., Mohr, S. E., Hu, Y., & Perrimon, N. (2021). Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos. Nature Communications, 12(1).
O’Leary, S., & Adelman, Z. N. (2020). CRISPR/Cas9 knockout of female-biased genes AeAct-4 or myo-fem in AE. Aegypti results in a flightless phenotype in female, but not male mosquitoes. PLOS Neglected Tropical Diseases, 14(12).
Liu, T., Yang, W., Xie, Y., Liu, P., Xie, L., Lin, F., Li, C., Gu, J., Wu, K., Yan, G., & Chen, X. (2018). Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus. Insect Science, 26(6), 1045–1054.
Alphey, L. S., Crisanti, A., Randazzo, F. (Fil), & Akbari, O. S. (2020). Standardizing the definition of Gene Drive. Proceedings of the National Academy of Sciences, 117(49), 30864–30867.
Kyrou, K., Hammond, A. M., Galizi, R., Kranjc, N., Burt, A., Beaghton, A. K., Nolan, T., & Crisanti, A. (2018). A CRISPR–cas9 gene drive targeting doublesex causes complete population suppression in caged anopheles gambiae mosquitoes. Nature Biotechnology, 36(11), 1062–1066
Simoni, A., Hammond, A. M., Beaghton, A. K., Galizi, R., Taxiarchi, C., Kyrou, K., Meacci, D., Gribble, M., Morselli, G., Burt, A., Nolan, T., & Crisanti, A. (2020). A male-biased sex-distorter gene drive for the human malaria vector anopheles gambiae. Nature Biotechnology, 38(9), 1054–1060.
Niu, G., Franc̨a, C., Zhang, G., Roobsoong, W., Nguitragool, W., Wang, X., Prachumsri, J., Butler, N. S., & Li, J. (2017). The fibrinogen-like domain of FREP1 protein is a broad-spectrum malaria transmission-blocking vaccine antigen. Journal of Biological Chemistry, 292(28), 11960–11969.
Dong, Y., Simões, M. L., Marois, E., & Dimopoulos, G. (2018). CRISPR/Cas9 -mediated gene knockout of anopheles gambiae FREP1 suppresses malaria parasite infection. PLOS Pathogens, 14(3).
Hoermann, A., Habtewold, T., Selvaraj, P., Del Corsano, G., Capriotti, P., Inghilterra, M. G., Kebede, T. M., Christophides, G. K., & Windbichler, N. (2022). Gene drive mosquitoes can aid malaria elimination by retarding plasmodium sporogonic development. Science Advances, 8(38).
Bansal, A., Molina-Cruz, A., Brzostowski, J., Liu, P., Luo, Y., Gunalan, K., Li, Y., Ribeiro, J. M., & Miller, L. H. (2018). PfCDPK1 is critical for malaria parasite gametogenesis and Mosquito infection. Proceedings of the National Academy of Sciences, 115(4), 774–779.
Macias, V. M., McKeand, S., Chaverra-Rodriguez, D., Hughes, G. L., Fazekas, A., Pujhari, S., Jasinskiene, N., James, A. A., & Rasgon, J. L. (2020). Cas9-mediated gene-editing in the malaria mosquito anopheles stephensi by remot control. G3&Amp;#58; Genes|Genomes|Genetics, 10(4), 1353–1360.
Garrood, W. T., Kranjc, N., Petri, K., Kim, D. Y., Guo, J. A., Hammond, A. M., Morianou, I., Pattanayak, V., Joung, J. K., Crisanti, A., & Simoni, A. (2021). Analysis of off-target effects in CRISPR-based gene drives in the human malaria mosquito. Proceedings of the National Academy of Sciences, 118(22).
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