A Mini Summary of Affinity-based Separation Methods for Sample Preparation

Authors

  • Yuming Chen

DOI:

https://doi.org/10.54097/hset.v36i.5669

Keywords:

Affinity-based, Sterility, Sample preparation, Cell separation.

Abstract

Over the years of development, affinity-based separation has become one of the main technologies of cell separation. These methods have met the requirements of high yield, purity, extensibility, and sterility. The techniques for separation of particles and cell purification which are applied in sample preparation in the field of basic scientific research and diagnostic or therapeutic purposes have become highly developed over years. In this paper, the common modern methods of affinity-based separation are discussed and compared, including their principles, fields of application, and limitations.

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References

Kaitlyn Bacon, Ashton Lavoie, Balaji M. Rao, Michael Daniele, Stefano Menegatti, “Past, Present, and Future of Affinity-based Cell Separation Technologies”, pp. 2 - 14, 2020.

Sarkar, A., Hou, H., Mahan, A. et al. Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics. Sci Rep 6, 23589 (2016).

Annette C Moser and David S Hage, “Immunoaffinity chromatography: an introduction to applications and recent developments”, Bioanalysis. 2010 Apr; 2 (4): 769 – 790.

Sabrina Kayo, Janina Bahnemann, Matthias Klauser, Ralf Pörtnera, and An-Ping Zeng, “A microfluidic device for immuno-affinity-based separation of mitochondria from cell culture”, Lap on a Chip, Issue 22, 2013.

Bayer, E. A. and Wilchek, M. (1990) The application of avidin-biotin technology for affinity-based separations. J. Chromatography 510, 5 – 11.

Livnah, O; Bayer, EA; Wilchek, M; Sussman, JL (1993). "Three-dimensional structures of Avidin and the Avidin-biotin complex". Proceedings of the National Academy of Sciences of the United States of America. 90 (11): 5076 – 80.

D. Mattanovich, N. Borth, Applications of cell sorting in biotechnology, Microbial cell factories 5 (1) (2006) 12.

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Published

21-03-2023