A Study of Multienzyme in MOF for Biosensing
DOI:
https://doi.org/10.54097/hset.v36i.6219Keywords:
Biosensors, Metal-organic-framework, Zeolitic Imidazole Framework-L, Enzymes.Abstract
At present, diabetes has become a widespread disease between young people and the elderly. New methods to detect diabetes are required. Hence, enzyme biosensors (glucose biosensors) based on immobilization methods are invented to solve the problem. However, enzyme stability can not be guaranteed. Metal-organic-framework has indicated a bright future in improving stability. In this project, o-Phenylenediamine (OPD) coating is used as a coloring agent to detect glucose. As is proved by previous researchers, taking advantage of the cascade reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP) enzymes, filter paper-based zeolitic imidazole framework-L (ZIF-L) thin film can be used to detect glucose considering its high acid stability and ease of fabrication. The results reveal that ZIF-L/GOx&HRP was linearly sensitive in the range of glucose concentrations (1, 2, 3, 4, and 5mM). After optimization by enzyme concentration and synthesis, 0.75mol/mL GOx&HRP with 30 minutes synthesis time illustrates the best performance.
Downloads
References
Akyilmaz E, Yorganci E, Asav E., et al. Do copper ions activate tyrosinase enzyme? A biosensor model for the solution [J]. Bioelectrochemistry, 2010, 78 (2): 155 - 160.
Diviés C., et al. Remarks on ethanol oxidation by an "Acetobacter xylinum" microbial electrode (author's transl) [J]. Annals of Microbiology, 1975, 126 (2): 175 - 186.
Rechnitz GA. Biochemical electrodes uses tissues slice [J]. Chemical & Engineering News, 2004, 56 (41): 16.
Keith C. Brown, John F. Corbett, Norman P. Loveless Spectrophotometric studies on the protonation of hydroxy and aminophenazines in aqueous solution [J]. Spectrochimica Acta Part A: Molecular Spectroscopy, 1979, 35 (5): 421 - 423.
Joseph Wang Glucose biosensors: 40 years of advances and challenges [J]. Electroanalysis, 2001, 13 (12): 983 - 988.
Stuart R. Batten, Neil R. Champness, Xiao-Ming Chen, et al. Terminology of metal–organic frameworks and coordination polymers (IUPAC Recommendations 2013 [J]. Pure and Applied Chemistry, 2013, 85 (8): 1715 - 1724.
Cejka J Metal-Organic Frameworks Applications from Catalysis to Gas Storage [B]., 2011.
Côté AP, Benin AI, Ockwig NW, et al. Porous, crystalline, covalent organic frameworks [J]. Science, 310 (5751): 1166 - 1170.
Effrosyni Gkaniatsou, Clémence Sicard, Rémy Ricoux, et al. Metal–organic frameworks: a novel host platform for enzymatic catalysis and detection [J]. Materials Horizons, 2007, 7: 55 - 63.
Mohammad, M., Razmjou, A., Liang, K., et al. Metal–Organic-Framework-Based Enzymatic Microfluidic Biosensor via Surface Patterning and Biomineralization [J]. ACS Applied Materials & Interfaces, 2019, 11 (2): 1807 - 1820.
Song G, Jingwei H, Zeyu D, et al. Improving the Acidic Stability of Zeolitic Imidazolate Frameworks by Biofunctional Molecules [J]. Chem, 2019, 5 (6): 1597 - 1608.
Liang W, Xu H, Carraro F, et al. Enhanced Activity of Enzymes Encapsulated in Hydrophilic Metal-Organic Frameworks [J]. Journal of the American Chemical Society, 2019, 141 (6): 2348 - 2355.
Peter R. Griffiths, James A. De Haseth, et al. Fourier Transform Infrared Spectrometry, 2nd Edition [B]. 2007.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
