Application And Comparative Analysis In SARS-Cov2 by PCR And CRISPR/Cas9

Abstract

COVID-19, a global large-scale epidemic caused by the novel coronavirus, is detrimental to people’s mental and physical health, and it also destruct the economic environment. In the process of the study of COVID-19, fast and accurate detective tools are necessary. There are plenty of clinical and laboratorial methods for detection. Nevertheless, due to various of realistic limitations, these methods cannot be fully applied clinically. This paper reviewed the some kinds of current polymerase chain reaction (PCR) technologies and the latest CRISPR/Cas9 in the detection of SARS-Cov2, compared the differences among these methods, and summarized the major achievements in cutting edge and limitations. In order to provide new ways to diagnose and cure the disease efficiently, and help doctors and researchers to investigate the complex pandemic and control the diffusion. PCR technology is the golden standard in the field of detecting COVID-19, while the high false negative rate and high requirements need to be improved. Many detective methods have been developed based on the CRISPR/Cas9 technology, each has its advantages, but also limited. In the future, the existing technology will be improved to explore the methods of mutual reference and combination between technologies, and find ways to improve the diagnostic efficiency and help clinical application, so as to provide help for the management of COVID-19 infection.