Bioinformatics Analysis of Core Genes Related to Sarcopenia
DOI:
https://doi.org/10.54097/j11jdm92Keywords:
Sarcopenia, Nosogenesis, Core Gene, BioinformaticsAbstract
Objective: To analyze the genes related to the pathogenesis of sarcopenia (SA) and find the potential therapeutic targets. Methods: GSE1428 data set downloaded from GEO gene expression omnibus, GEO) was taken as the analysis object. The data were standardized by R software, and the differential genes were screened. The GO and KEGG function enrichment analysis of the differential genes was carried out by Metascape, and the PPI analysis was carried out by String. The results were imported into Cytoscape software to screen out the core genes, which were imported into BioGPS database for tissue localization. Results: 117 differential genes were obtained, including 54 down-regulated genes and 63 up-regulated genes. GO and KEGG analysis showed that GO was mainly enriched in ethanol reaction, carboxylic acid transport, cell secretion regulation, vitamin metabolism, leukocyte chemotaxis regulation and bone marrow cell differentiation regulation, while KEGG was mainly enriched in viral carcinogenesis pathway. There are 108 nodes and 44 connections in the PPI network. According to the PPI results, Cytoscape screened out core genes such as RPE65, TOPORS, IMPG2 and IMPDH1, among which RPE65, TOPORS and IMPDH1 were down-regulated and IMPG2 was up-regulated. The localization of gene tissue suggested that RPE65 was located in retina. Conclusion: Screening the core genes and related signal pathways is helpful to understand the pathogenesis of SA and provide new ideas for drug therapy research of SA.
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References
Kim YC, Ki SW, Kim H, Kang S, Kim H, Go GW. Recent Advances in Nutraceuticals for the Treatment of Sarcopenic Obesity. Nutrients. 2023;15(17):3854.
CAO L, MORLEY J E. Sarcopenia Is Recognized as an Independent Condition by an International Classification of Disease, Tenth Revision, Clinical Modification (ICD-10-CM) Code[J]. J Am Med Dir Assoc, 2016,17(8): 675-677.
HAN P, KANG L, GUO Q, et al. Prevalence and Factors Associated With Sarcopenia in Suburb-dwelling Older Chinese Using the Asian Working Group for Sarcopenia Definition[J]. J Gerontol A Biol Sci Med Sci, 2016,71(4): 529-535.
LIU P, HAO Q, HAI S, et al. Sarcopenia as a predictor of all-cause mortality among community-dwelling older people: A systematic review and meta-analysis[J]. Maturitas, 2017,103: 16-22.
KIRK B, AL S A, DUQUE G. Osteosarcopenia: A case of geroscience[J]. Aging Med (Milton), 2019,2(3): 147-156.
CRUZ-JENTOFT A J, SAYER A A. Sarcopenia[J]. Lancet, 2019,393(10191): 2636-2646.
GOLJANEK-WHYSALL K, IWANEJKO L A, VASILAKI A, et al. Ageing in relation to skeletal muscle dysfunction: redox homoeostasis to regulation of gene expression[J]. Mamm Genome, 2016,27(7-8): 341-357.
PELLEGRINO A, TIIDUS P M, VANDENBOOM R. Mechanisms of Estrogen Influence on Skeletal Muscle: Mass, Regeneration, and Mitochondrial Function[J]. Sports Med, 2022,52(12): 2853-2869.
PAN L, XIE W, FU X, et al. Inflammation and sarcopenia: A focus on circulating inflammatory cytokines[J]. Exp Gerontol, 2021,154: 111544.
Chechenova M, Stratton H, Kiani K, et al. Quantitative model of aging-related muscle degeneration: a Drosophila study. Preprint. bioRxiv. 2023;2023.02.19.529145.
CHUNG T H, SHIM J Y, LEE Y J. Association between leukocyte count and sarcopenia in postmenopausal women: The Korean National Health and Nutrition Examination Survey [J]. Maturitas, 2016,84: 89-93.
Im YG, Han MY, Baek HS. Association of Serum Vitamin D Level with Temporomandibular Disorder Incidence: A Retrospective, Multi-Center Cohort Study Using Six Hospital Databases. Nutrients. 2023;15(13):2860.
Papadopoulou SK. Sarcopenia: A Contemporary Health Problem among Older Adult Populations. Nutrients. 2020;12 (5): 1293.
Conte E, Dinoi G, Imbrici P, De Luca A, Liantonio A. Sarcoplasmic Reticulum Ca2+ Buffer Proteins: A Focus on the Yet-To-Be-Explored Role of Sarcalumenin in Skeletal Muscle Health and Disease. Cells. 2023;12(5):715.
Li Q, Ishii KA, Kamoshita K, et al. PAC1 Deficiency Protects Obese Male Mice From Immobilization-Induced Muscle Atrophy by Suppressing FoxO-Atrogene Axis. Endocrinology. 2023;164(6): bqad065.
PEI J, GRISHIN N V. Expansion of divergent SEA domains in cell surface proteins and nucleoporin 54[J]. Protein Sci, 2017,26(3): 617-630.
CHOI J Y, LOPES L, BEN M C, et al. Maturation of the malarial phosphatidylserine decarboxylase is mediated by high affinity binding to anionic phospholipids[J]. J Biol Chem, 2023, 299(5): 104659.
KEPPEKE G D, ANDRADE L, BARCELOS D, et al. IMPDH-Based Cytoophidium Structures as Potential Theranostics in Cancer[J]. Mol Ther, 2020,28(7): 1557-1558.
RUAN H, SONG Z, CAO Q, et al. IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors[J]. Mol Ther, 2020,28(5): 1299-1313.
LIU C, ZHANG W, ZHOU X, et al. IMPDH1, a prognostic biomarker and immunotherapy target that correlates with tumor immune microenvironment in pan-cancer and hepatocellular carcinoma[J]. Front Immunol, 2022,13: 983490.
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