Screening Anthraquinones by Fluorescent Ubiquitination Cell Cycle Indicators

Yan Zheng; Lirong Wang; Jingqi Yang; Shihua Mei; Qingzhen Peng; Rong Zhang

doi:10.54097/jk1q1558

Authors

Yan Zheng
Lirong Wang
Jingqi Yang
Shihua Mei
Qingzhen Peng
Rong Zhang

DOI:

https://doi.org/10.54097/jk1q1558

Keywords:

Cell Cycle, FUCCI, Fluorescent Protein, Real-time Observation, Cell Cycle Factor, Tumor Cell

Abstract

Cancer is one of the diseases with the highest mortality rate in the world, and its occurrence and development are mainly related to the uncontrolled cell cycle. The cell cycle is the completion of a cell division from the end of the n1ext division, the cell cycle is divided They are interphase and division phase. Specifically, there's a DNA synthesis phase (G1 phase), DNA synthesis phase (S phase), and DNA synthesis phase (G2 phase) Phase) and division phase (M phase). Tumor cells can proliferate indefinitely, mainly with the cell cycle getting out of control, making it not subject to normal Growth regulation. The current methods for detecting cell cycle mainly include: Acridine Orange (AO) method, 5-Bromo-2-deoxy Uridine (BrdU) labeling method, Western blot and flow cytometry, etc., but these methods have certain limitations, such as the inability to make real-time observations in living cells. Therefore, we constructed the Cell Cycle Ubiquitination-based Cell Cycle Indicator (FUCCI) tool in liver cancer cells, hoping to provide a new technical means for the screening of anti-tumor drugs.

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