Progress and prospect of protein detection methods based on CRISPR-Cas system
DOI:
https://doi.org/10.54097/hset.v66i.11713Keywords:
CRISPR-Cas, protein detection, fluorescence, clinical diagnosis, bioresearch.Abstract
This is a review article on protein detection methods based on CRISPR-Cas system. This article introduces the application of CRISPR-Cas system in the field of gene editing, and then focuses on the protein detection technology based on CRISPR-Cas system. These technologies take advantage of the targeting and high efficiency of the CRISPR-Cas system, combined with detection methods such as fluorescence or mass spectrometry, to detect a variety of proteins with high sensitivity. This article also compares the advantages and disadvantages of these techniques and traditional protein detection methods, and discusses the application prospects of these techniques in clinical diagnosis and biological research.
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References
Mincu, N.B., et al., Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose. DIAGNOSTICS, 2020. 10(8).
Zhou, S.S., et al., Review: Multiplexed profiling of biomarkers in extracellular vesicles for cancer diagnosis and therapy monitoring. ANALYTICA CHIMICA ACTA, 2021. 1175.
Shin, J., M. Miller and Y.C. Wang, Recent advances in CRISPR-based systems for the detection of foodborne pathogens. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2022. 21(3): p. 3010-3029.
Mons, E., R.Q. Kim and M. Mulder, Technologies for Direct Detection of Covalent Protein-Drug Adducts. PHARMACEUTICALS, 2023. 16(4).
Cohen, L. and D.R. Walt, Highly Sensitive and Multiplexed Protein Measurements. CHEMICAL REVIEWS, 2019. 119(1): p. 293-321.
Liu, G.Y., et al., Recent Advances on Fluorescence-based Enzyme Linked Immunosorbent Assay. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2023. 51(3): p. 331-339.
Xu, Z., et al., A enzyme-linked immunosorbent assay based on metal-organic frameworks (MOFs) for sensitive detection of aflatoxin B-1. FOOD CHEMISTRY, 2021. 338.
Ghosh, R., J.E. Gilda and A.V. Gomes, The necessity of and strategies for improving confidence in the accuracy of western blots. EXPERT REVIEW OF PROTEOMICS, 2014. 11(5): p. 549-560.
Reichel, C., G. Gmeiner and M. Thevis, Antibody-based strategies for the detection of Luspatercept (ACE-536) in human serum. DRUG TESTING AND ANALYSIS, 2017. 9(11-12): p. 1721-1730.
Cui, M., C. Cheng and L. Zhang, High-throughput proteomics: a methodological mini-review. Laboratory investigation; a journal of technical methods and pathology, 2022. 102(11): p. 1170-1181.
Maes, E., et al., The benefits and limitations of reaction cell and sector field inductively coupled plasma mass spectrometry in the detection and quantification of phosphopeptides. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2015. 29(1): p. 35-44.
Li, Y.Y., et al., Amplified detection of nucleic acids and proteins using an isothermal proximity CRISPR Cas12a assay. CHEMICAL SCIENCE, 2021. 12(6): p. 2133-2137.
Wang, J., et al., Recent Advances in CRISPR/Cas-Based Biosensors for Protein Detection. BIOENGINEERING-BASEL, 2022. 9(10).
Dolgalev, G. and E. Poverennaya, Applications of CRISPR-Cas Technologies to Proteomics. GENES, 2021. 12(11).
Gonzalez-Salinas, F., C. Martinez-Amador and V. Trevino, Characterizing genes associated with cancer using the CRISPR/Cas9 system: A systematic review of genes and methodological approaches. GENE, 2022. 833.
Jiang, L.Y., et al., Advances in gene therapy hold promise for treating hereditary hearing loss. MOLECULAR THERAPY, 2023. 31(4): p. 934-950.
Hussen, B.M., et al., Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges. MILITARY MEDICAL RESEARCH, 2023. 10(1).
Das, N., D.G. Dhar and P. Dhar, Editing the genome of common cereals (Rice and Wheat): techniques, applications, and industrial aspects. MOLECULAR BIOLOGY REPORTS, 2023. 50(1): p. 739-747.
Zetsche, B., et al., Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. CELL, 2015. 163(3): p. 759-771.
Abudayyeh, O.O., et al., C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. SCIENCE, 2016. 353(6299).
Gootenberg, J.S., et al., Nucleic acid detection with CRISPR-Cas13a/C2c2. SCIENCE, 2017. 356(6336): p. 438-+.
Chen, J.S., et al., CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. SCIENCE, 2018. 360(6387): p. 436-+.
Gootenberg, J.S., et al., Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. SCIENCE, 2018. 360(6387): p. 439-+.
Barber, K.W., E. Shrock and S.J. Elledge, CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays. MOLECULAR CELL, 2021. 81(17): p. 3650-+.
Yi, W.K., et al., CRISPR-assisted detection of RNA-protein interactions in living cells. NATURE METHODS, 2020. 17(7): p. 685-+.
Yu Yanyan et al. PPIs detection platform and detection method based on CRISPR-Cas12a sensing system,. 2022.
Peterson, B.S., et al., Remodeling of the Acetylproteome by SIRT3 Manipulation Fails to Affect Insulin Secretion or beta Cell Metabolism in the Absence of Overnutrition. CELL REPORTS, 2018. 24(1): p. 209-+.
Choi, J.H., et al., CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis. NANO LETTERS, 2021. 21(1): p. 693-699.
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