Observational study of blood complement levels of components before infusion

Abstract

To investigate the changes in complement content in component blood before clinical transfusion. According to different preparation processes, storage temperatures and time periods, 180 specimens of plasma from the same source of 20 people were left at different stages of the preparation process. Homologous specimens were concentrated in the same batch of experiments to observe the changes in complement C3 and C4 content under different storage temperature and process stage grouping conditions. Using the same specimen to detect C3 and C4 content, the detection results of homologous specimens were sorted into nine groups according to different storage temperatures and preparation process stages for comparison.and there is no significant difference between simple -40°C and -60°C storage at different temperatures. Storage at 4°C for 7 days can significantly reduce complement content. Virus inactivation preparation process can significantly reduce complement content. Repeated freezing and thawing during the processing of frozen plasma can change complement content. Correlation analysis of complement C3 and C4 content determination experiments showed that the changes of the two under different conditions such as temperature, preparation process and storage time were significantly positively correlated. The storage temperature and storage time of frozen plasma, repeated freezing and thawing, centrifugal concentration and light inactivation can affect the complement content.