Optimization of Extraction Process and Preliminary Study on Hypoglycemic Activity of Fructus Swietenia Macrophylla Extract
DOI:
https://doi.org/10.54097/0te7fg92Keywords:
Fructus Swietenia Macrophylla (FSM), Extraction process, Amylase, inhibition, Hypoglycemic effect.Abstract
This project studies the method of ultrasonic-assisted organic solvent extraction to extract hypoglycemic components from Fructus Swietenia Macrophylla. It explores the effects of different solvents, ultrasonic time, solid-liquid ratio, extraction temperature, and ultrasonic frequency on the extraction rate of FSM extract. Single-factor experiments and orthogonal experiments are used to optimize the extraction process, and the extract yield is used as an indicator to evaluate the advantages and disadvantages of the process. With the α-Amylase and β-The inhibition rate of amylase is used as an indicator to evaluate the hypoglycemic activity of the above-mentioned FSM extract. The results showed that using ethyl acetate as the extraction solvent, ultrasonic time of 30 mins, solid-liquid ratio of 1:25 (m/V), ultrasonic frequency of 40 kHz, and ultrasonic temperature of 50℃ were the optimal extraction process for FSM extract. When adding 10 mg of FSM extract α-The inhibition rate of amylase can reach 78.74% ± 1.8%, which is beneficial for β-The inhibition rate of amylase can reach 85.12% ± 2.38%, indicating a good inhibitory effect, indicating that FSM extract has certain hypoglycemic activity.
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References
Da Ju, J.M. Garg. Xiang Tianguo [J]. Global Human Geography, 2017 (01): 115.
Zhang Jun. Xiang Tianguo - A Magic Fruit for Maintaining Health [J]. Chinese Journal of Health and Nutrition, 2004 (04): 77-78.
Julie Study on the Chemical Constituents of FSM [D]. Hefei: Anhui University of Traditional Chinese Medicine, 2015.
Wu Weihong, Li Enchao, Zhou Fujia, et al. GC-MS analysis of petroleum ether extracts from FSM [J]. Journal of Xuzhou Medical University, 2019, 39 (09): 634-637.
Su Jianhui Study on the hypoglycemic and lipid-lowering activities and mechanisms of peony seed oil and its compound [D]. Wuxi: Jiangnan University, 2016.
Liu Jinsong, Zhu Lili, Wang Guokai, et al. Study on the Chemical Constituents of Xiangtianguo [J] Traditional Chinese Medicine, 2016, 39 (3): 1530-1535.
Zhang Qiaoxiang, Luo Yaoquan, Xu Tao, et al. Experimental Study on the Effect of Xiangtianguo Extract on Renal Hypertension in Animals [J] Progress in Animal Medicine, 2019, 40 (1): 1-71.
Ma YQ, Liu MH, Jiang K, Guo L, Qu SJ, Wan YQ, Tan CH. Limonoids from the fruits of Swietenia macrophylla with inhibitory activity against H2O2-induced apoptosis in HUVECs [J]. Fitoterapia. 2018;129:179-184.
Cheng YB, Chien YT, Lee JC, Tseng CK, Wang HC, Lo IW, Wu YH, Wang SY, Wu YC, Chang FR. Limonoids from the seeds of Swietenia macrophylla with inhibitory activity against dengue virus 2 [J]. J Nat Prod. 2014;77(11):2367-74.
Jih Jung Chen, Sheng-Shiou Huang, Chang-Hui Liao, Dau-Chang Wei, Ping-Jyun Sung, Tai-Chi Wang, Ming-Jen Cheng. A new phragmalin-type limonoid and anti-inflammatory constituents from the fruits of Swietenia macrophylla [J]. Food Chemistry. 2010. 120(2):379-384.
Arumugasamy, K., Latha, K. V., Kumar, N. H. Studies on some pharmacognostic profiles of swietenia macrophylla. King [J]. Anc Sci Life, 2004, 26(2):97-102.
Zhang QX, Zhang JN, Wang WS, et al. Research progress on pharmacological activity of FSM [C]. Chinese Society of Laboratory Zoology. Proceedings of the 13th Annual Conference of Chinese Laboratory Animal Science. Experimental Animal Center of Southern Medical University, 2017: 5.
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