Progress of Construction of Gene-Modified Soybean Based on the CRISPR/Cas9 System
DOI:
https://doi.org/10.54097/1j3ams13Keywords:
CRISPR/Cas9, soybean, gene editing.Abstract
From the first discovery by Japanese scholar Ishino in 1987 to being named and classified in 2002, CRISPR technology was proven by the Nobel Prize for Chemistry in 2020. In 2010, the type II CRISPR/Cas system (CRISPR/Cas9) was developed as an RNA-targeted genome editing system, which, as a novel and emerging genome editing technology in recent years, has a promising application prospect because of its high efficiency, simplicity and low cost. There is a serious mismatch between the plant area and yield of soybeans in China with market demand. Soybean is an important source of oil and biofuel. It is closely related to national food security. The large demand has led to a high dependence on imports of soybeans in China. Therefore, there is an urgent need to breed Chinese soybean varietal strains with outstanding forms in soybean selection and breed in order to breeding good strains and realize the goal of basically satisfying independent production. This review summarizes the research of the CRISPR/Cas9 system in constructing biological models of specific genes or genomic deletions in soybeans, introduces the latest development of this technology, and discusses for the advantages of CRISPR/Cas9 system in breeding target soybeans with good resistance and high economic production value.
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